標題: Immunohistochemical analysis of a novel dehydroepiandrosterone sulfotransferase-like protein in Drosophila neural circuits
作者: Liu, Tzu-An
Liu, Ming-Cheh
Yang, Yuh-Shyong
生物科技學系
Department of Biological Science and Technology
關鍵字: sulfation;Drosophila;DHEA ST;memory;neuropharmacology
公開日期: 29-Feb-2008
摘要: Sulfotransferase (ST)-catalyzed sulfation plays an important role in various neuronal functions such as homeostasis of catecholamine neurotransmitters and hormones. Drosophila is a popular model for the study of memory and behavioral manifestations because it is able to mimic the intricate neuroregulation and recognition in humans. However, there has been no evidence indicating that cytosolic ST(s) is(are) present in Drosophila. The aim of this study is to investigate whether or not cytosolic ST(s) is(are) expressed in the Drosophila nervous system. Immunoblot analysis demonstrated the presence of dehydroepiandrosterone (DHEA) ST-like protein in Drosophila brain and a sensitive fluorometric assay revealed its sulfating activity toward DHEA. Immunohistochemical staining demonstrated this DHEA ST-like protein to be abundant in specific neurons as well as in several bundles of nerve fibers in Drosophila. Clarification of a possible link between ST and a neurotransmitter-mediated effect may eventually aid in designing approaches for alleviating neuronal disorders in humans. (C) 2007 Elsevier Inc. All rights reserved.
URI: http://dx.doi.org/10.1016/j.bbrc.2007.12.082
http://hdl.handle.net/11536/9650
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2007.12.082
期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume: 367
Issue: 1
起始頁: 14
結束頁: 20
Appears in Collections:Articles


Files in This Item:

  1. 000252619700003.pdf