標題: A new approach for quantitative determination of glucose by using CdSe/ZnS quantum dots
作者: Huang, Chin-Ping
Liu, Shu-Wei
Chen, Teng-Ming
Li, Yaw-Kuen
應用化學系
Department of Applied Chemistry
關鍵字: assay;quantum dots;photoluminescence;glucose determination
公開日期: 14-三月-2008
摘要: Quantitative detection of glucose is an important issue in food industry, various biotechnological processes and medication of metabolic disorders. Many ways have been developed to approach this target. In this study, we demonstrated a simple assay system containing glucose oxidase (GOD) and CdSe/ZnS quantum dots (QDs) for specific and quantitative detection of glucose, The role of QDs functioned as an "indicator" to reveal the acidity change of the assay system resulting from the enzymatic oxidation of glucose. By controlling the initial buffer conditions of the assay system at 10 mM or 30 mM phosphate, pH 8.0, the quench ratio of PL intensity of QDs at 586 nm was found to be linear to the tested glucose in the range of 0.2-10 mM or 2-30 mM, respectively. The assay system also provides a convenient way to determine the glucose concentration by visuahzing the color change of QDs fluorescence. As compare to most of the existing methods, this newly developed system possesses many advantages, including simplicity, low cost, high flexibility, and good sensitivity. Furthermore, no complicated chemical modification and enzyme immobilization were needed in this system. (C) 2007 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.snb.2007.08.021
http://hdl.handle.net/11536/9574
ISSN: 0925-4005
DOI: 10.1016/j.snb.2007.08.021
期刊: SENSORS AND ACTUATORS B-CHEMICAL
Volume: 130
Issue: 1
起始頁: 338
結束頁: 342
顯示於類別:期刊論文


文件中的檔案:

  1. 000254681900050.pdf