標題: Using oligonucleotide-functionalized Au nanoparticles to rapidly detect foodborne pathogens on a piezoelectric biosensor
作者: Chen, Sz-Hau
Wu, Vivian C. H.
Chuang, Yao-Chen
Lin, Chih-Sheng
生物科技學系
Department of Biological Science and Technology
關鍵字: Au nanoparticle;piezoelectric biosensor;E. coli O157 : H7
公開日期: 1-Apr-2008
摘要: A circulating-flow piezoelectric biosensor, based on an Au nanoparticle amplification and verification method, was used for real-time detection of a foodbome pathogen, Escherichia coli O157:H7. A synthesized thiolated probe (Probe 1; 30-mer) specific to E. coli O157:H7 eaeA gene was immobilized onto the piezoelectric biosensor surface. Hybridization was induced by exposing the immobilized probe to the E. coli O157:H7 eaeA gene fragment (104-bp) amplified by PCR, resulting in a mass change and a consequent frequency shift of the piezoelectric biosensor. A second thiolated probe (Probe 2), complementary to the target sequence, was conjugated to the An nanoparticles and used as a "mass enhancer" and "sequence verifier" to amplify the frequency change of the piezoelectric biosensor. The PCR products amplified from concentrations of 1.2 x 10(2) CFU/ml of E. coli O157:H7 were detectable by the piezoelectric biosensor. A linear correlation was found when the E. coli O157:H7 detected from 10(2) to 10(6) CFU/ml. The piezoelectric biosensor was able to detect targets from real food samples. (C) 2008 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.mimet.2008.01.004
http://hdl.handle.net/11536/9478
ISSN: 0167-7012
DOI: 10.1016/j.mimet.2008.01.004
期刊: JOURNAL OF MICROBIOLOGICAL METHODS
Volume: 73
Issue: 1
起始頁: 7
結束頁: 17
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