標題: Fabrication of a carbon fiber paper as the electrode and its application toward developing a sensitive unmediated amperometric biosensor
作者: Yuan, Chiun-Jye
Wang, Chung-Liang
Wu, Teng Yang
Hwang, Kuo-Chu
Chao, Wei-Chi
生物科技學系
分子醫學與生物工程研究所
Department of Biological Science and Technology
Institute of Molecular Medicine and Bioengineering
關鍵字: Amperometric biosensor;Carbon fiber paper;Nitrogen plasma;Phenolic compounds;Tyrosinase
公開日期: 15-Feb-2011
摘要: Carbon fiber paper (CFP), a material frequently used as the diffusion layer in fuel cells, was found recently to exhibit a potential as an electrode for the development of sensitive, unmediated biosensors. After nitrogen plasma treatment, the CFP exhibited a quasi-reversible behavior to the redox couple (e.g., ferricyanide) with an electron transfer rate constant of 7.2 x 10(-3) cm s(-1). This rate constant is approximately double that of a Pt-electrode and is much higher than that of many carbon-based electrodes. The unmediated CFP-based tyrosinase biosensor fabricated for this study exhibited an optimal working potential and operating pH value of -0.2 V and 6.5, respectively. Compared to other unmediated tyrosinase biosensors, the CFP-based tyrosinase biosensor offers a high sensitivity for the monitoring of phenolic compounds (17.8, 7.1, 5.2 and 3.7 mu A mu M(-1) cm(-2) for catechol, phenol, bisphenol and 3-aminophenol, respectively). The lowest detection limit for catechol, phenol, bisphenol and 3-aminophenol was 2, 5, 5 and 12 nM, respectively. Furthermore, this biosensor exhibited a good repeatability, a fast response time (around 105), and a wide linear dynamic range of detection for phenolic compounds. (C) 2010 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.bios.2010.11.023
http://hdl.handle.net/11536/9300
ISSN: 0956-5663
DOI: 10.1016/j.bios.2010.11.023
期刊: BIOSENSORS & BIOELECTRONICS
Volume: 26
Issue: 6
起始頁: 2858
結束頁: 2863
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