標題: Helicobacter pylori induces gene expression in human gastric cells identified by mRNA differential display
作者: Wong, L
Lue, MY
Chang, CA
Lin, YL
Chan, EC
生物科技學系
Department of Biological Science and Technology
公開日期: 12-十一月-1996
摘要: Changes in gene expression patterns in gastric cells infected with Helicobacter pylori were characterized by means of mRNA differential display. Total RNA preparations were extracted from the H. pylori infected gastric cells and paired non-infected cells, and were probed with candidate clones identified after screening up to 6,000 mRNA species. Among them, four clones, 04G-1, 04G-2, 01G-1, and Cppa-2 show significant expression in the infected cells by Northern blot analysis, and they are 199 bp, 196 bp, 228 bp, and 276 bp in length, respectively. Database search revealed that nucleotide sequences of these clones share very low identity with any known sequence. These results indicate that H. pylori can significantly affect gene expression in gastric cells. Furthermore mRNA differential display can be used in pathogenesis studies to identify new genes in gastric cells in response to insults such as H. pylori. (C) 1996 Academic Press, Inc.
URI: http://dx.doi.org/10.1006/bbrc.1996.1686
http://hdl.handle.net/11536/927
ISSN: 0006-291X
DOI: 10.1006/bbrc.1996.1686
期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume: 228
Issue: 2
起始頁: 484
結束頁: 488
顯示於類別:期刊論文


文件中的檔案:

  1. A1996VT76800042.pdf