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dc.contributor.authorOkano, Kazunorien_US
dc.contributor.authorYu, Daviden_US
dc.contributor.authorMatsui, Aien_US
dc.contributor.authorMaezawa, Yasuyoen_US
dc.contributor.authorHosokawa, Yoichirohen_US
dc.contributor.authorKira, Atsushien_US
dc.contributor.authorMatsubara, Mieen_US
dc.contributor.authorLiau, Ianen_US
dc.contributor.authorTsubokawa, Hiroshien_US
dc.contributor.authorMasuhara, Hiroshien_US
dc.date.accessioned2014-12-08T15:11:54Z-
dc.date.available2014-12-08T15:11:54Z-
dc.date.issued2011-03-21en_US
dc.identifier.issn1439-4227en_US
dc.identifier.urihttp://dx.doi.org/10.1002/cbic.201000497en_US
dc.identifier.urihttp://hdl.handle.net/11536/9134-
dc.description.abstractThis article describes a novel laser-directed microfabrication method carried out in aqueous solution for the organization of cell networks on a platform. A femtosecond (fs) laser was applied to a platform culturing PC12, HeLa, or normal human astrocyte (NHA) cells to manipulate them and to facilitate mutual connections. By applying an fs-laser-induced impulsive force, cells were detached from their original location on the plate, and translocated onto microfabricated cell-adhesive domains that were surrounded with a cell-repellent perfluoroalkyl (R(f)) polymer. Then the fs-laser pulse-train was applied to the Rf polymer surface to modify the cell-repellent surface, and to make cell-adhesive channels of several mm in width between each cell-adhesive domain. PC12 cells elongated along the channels and made contact with others cells. HeLa and NHA cells also migrated along the channels and connected to the other cells. Surface analysis by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) confirmed that the Rf polymer was partially decomposed. The method presented here could contribute not only to the study of developing networks of neuronal, glial, and capillary cells, but also to the quantitative analysis of nerve function.en_US
dc.language.isoen_USen_US
dc.subjectartificial cell networksen_US
dc.subjectcell adhesionen_US
dc.subjectcell arraysen_US
dc.subjectlaser chemistryen_US
dc.subjectlithograhyen_US
dc.titleInduction of Cell-Cell Connections by Using in situ Laser Lithography on a Perfluoroalkyl-Coated Cultivation Platformen_US
dc.typeArticleen_US
dc.identifier.doi10.1002/cbic.201000497en_US
dc.identifier.journalCHEMBIOCHEMen_US
dc.citation.volume12en_US
dc.citation.issue5en_US
dc.citation.spage795en_US
dc.citation.epage801en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.department應用化學系分子科學碩博班zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.contributor.departmentInstitute of Molecular scienceen_US
dc.identifier.wosnumberWOS:000288563000020-
dc.citation.woscount8-
Appears in Collections:Articles


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