標題: Induction of Cell-Cell Connections by Using in situ Laser Lithography on a Perfluoroalkyl-Coated Cultivation Platform
作者: Okano, Kazunori
Yu, David
Matsui, Ai
Maezawa, Yasuyo
Hosokawa, Yoichiroh
Kira, Atsushi
Matsubara, Mie
Liau, Ian
Tsubokawa, Hiroshi
Masuhara, Hiroshi
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: artificial cell networks;cell adhesion;cell arrays;laser chemistry;lithograhy
公開日期: 21-Mar-2011
摘要: This article describes a novel laser-directed microfabrication method carried out in aqueous solution for the organization of cell networks on a platform. A femtosecond (fs) laser was applied to a platform culturing PC12, HeLa, or normal human astrocyte (NHA) cells to manipulate them and to facilitate mutual connections. By applying an fs-laser-induced impulsive force, cells were detached from their original location on the plate, and translocated onto microfabricated cell-adhesive domains that were surrounded with a cell-repellent perfluoroalkyl (R(f)) polymer. Then the fs-laser pulse-train was applied to the Rf polymer surface to modify the cell-repellent surface, and to make cell-adhesive channels of several mm in width between each cell-adhesive domain. PC12 cells elongated along the channels and made contact with others cells. HeLa and NHA cells also migrated along the channels and connected to the other cells. Surface analysis by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) confirmed that the Rf polymer was partially decomposed. The method presented here could contribute not only to the study of developing networks of neuronal, glial, and capillary cells, but also to the quantitative analysis of nerve function.
URI: http://dx.doi.org/10.1002/cbic.201000497
http://hdl.handle.net/11536/9134
ISSN: 1439-4227
DOI: 10.1002/cbic.201000497
期刊: CHEMBIOCHEM
Volume: 12
Issue: 5
起始頁: 795
結束頁: 801
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