Title: Structural and Mechanical Properties of Klebsiella pneumoniae Type 3 Fimbriae
Authors: Chen, Feng-Jung
Chan, Chia-Han
Huang, Ying-Jung
Liu, Kuo-Liang
Peng, Hwei-Ling
Chang, Hwan-You
Liou, Gunn-Guang
Yew, Tri-Rung
Liu, Cheng-Hsien
Hsu, Ken Y.
Hsu, Long
Department of Biological Science and Technology
Department of Electrophysics
Department of Photonics
Issue Date: 1-Apr-2011
Abstract: This study investigated the structural and mechanical properties of Klebsiella pneumoniae type 3 fimbriae, which constitute a known virulence factor for the bacterium. Transmission electron microscopy and optical tweezers were used to understand the ability of the bacterium to survive flushes. An individual K. pneumoniae type 3 fimbria exhibited a helix-like structure with a pitch of 4.1 nm and a three-phase force-extension curve. The fimbria was first nonlinearly stretched with increasing force. Then, it started to uncoil and extended several micrometers at a fixed force of 66 +/- 4 pN (n +/- 22). Finally, the extension of the fimbria shifted to the third phase, with a characteristic force of 102 +/- 9 pN (n = 14) at the inflection point. Compared with the P fimbriae and type 1 fimbriae of uropathogenic Escherichia coli, K. pneumoniae type 3 fimbriae have a larger pitch in the helix-like structure and stronger uncoiling and characteristic forces.
URI: http://dx.doi.org/10.1128/JB.01395-10
ISSN: 0021-9193
DOI: 10.1128/JB.01395-10
Volume: 193
Issue: 7
Begin Page: 1718
End Page: 1725
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