Title: 1064 nm Deep Near-Infrared (NIR) Excited Raman Microspectroscopy for Studying Photolabile Organisms
Authors: Ando, Masahiro
Sugiura, Miwa
Hayashi, Hidenori
Hamaguchi, Hiro-o
應用化學系
Department of Applied Chemistry
Keywords: Near-infrared Raman spectroscopy;Multichannel Raman spectroscopy;1064 nm excitation;Microspectroscopy;Cyanobacteria;Thermosynechococcus elongatus
Issue Date: 1-May-2011
Abstract: We have constructed a 1064 nm deep near-infrared (NIR) excited multichannel Raman microspectrometer using an InP/InGaAsP multichannel detector. This microspectrometer achieves high sensitivity suitable for in vivo measurements of single living cells with lateral resolution of 0.7 mu m and depth resolution of 3.1 mu m. It has been applied to the structural analysis of living cyanobacterial cells, well-known model organisms for photosynthesis research, which are too photolabile to be measured with visible laser excitation. High signal-to-noise ratio (SIN) Raman spectra have been obtained from carotenoid, chlorophyll a, and phycocyanin in a single living cyanobacterial cell with no appreciable interference from autofluorescence or photodamage. Sub-micrometer mapping of Raman intensities provides clear distribution images of the three pigments inside the cell.
URI: http://dx.doi.org/10.1366/10-06196
http://hdl.handle.net/11536/8958
ISSN: 0003-7028
DOI: 10.1366/10-06196
Journal: APPLIED SPECTROSCOPY
Volume: 65
Issue: 5
Begin Page: 488
End Page: 492
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