Title: An inverted method for culturing dissociated mouse hippocampal neurons
Authors: Chen, Wen-Shin
Yueh, Chao-Yen
Huang, Yung-An
Hwang, Eric
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
Institute of Molecular Medicine and Bioengineering
Keywords: Mouse hippocampal neuron;Primary culture;Dissociated neuron culture
Issue Date: 1-May-2011
Abstract: Dissociated hippocampal neuron culture has long been the model system of choice for many neuroscientists. The ability to culture dissociated hippocampal neurons from genetically modified mice provides an invaluable tool for studying many neuronal processes. In this study, we established a novel method to culture dissociated hippocampal neurons from embryonic and neonatal mice. Dissociated neurons were cultured in a microchamber between the glass coverslip and the plastic cell container without the use of glial feeder cells. Our method significantly simplifies the preparation while produces healthy and long-lived neuronal cultures that are difficult to achieve without the use of feeder cells. (C) 2011 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
URI: http://dx.doi.org/10.1016/j.neures.2011.01.002
ISSN: 0168-0102
DOI: 10.1016/j.neures.2011.01.002
Volume: 70
Issue: 1
Begin Page: 118
End Page: 123
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