標題: A Unique and Potent Protein Binding Nature of Liposome Containing Polyethylenimine and Polyethylene Glycol: A Nondisplaceable Property
作者: Liu, Yen-Ku
Lin, Yu-Ling
Chen, Chia-Hung
Lin, Ching-Min
Ma, Kuan-Lun
Chou, Fu-Hsuan
Tsai, Jin-Sheng
Lin, Hsin-Yu
Chen, Fu-Rong
Cheng, Tian-Lu
Chang, Chia-Ching
Liao, Kuang-Wen
生物科技學系
分子醫學與生物工程研究所
Department of Biological Science and Technology
Institute of Molecular Medicine and Bioengineering
關鍵字: liposomes;lipid coating;polyethylenimine;protein capturing;cell staining
公開日期: 1-六月-2011
摘要: Most of the currently available targeting vectors are produced via the linkage of targeting molecules. However, the coupling process is complicated, and the covalent linkage may attenuate the activity of certain targeting molecules. In this study, we have developed a cationic liposome complexed with polyethylenimine and polyethylene glycol polymers (LPPC) that can capture various proteins without covalent conjugation. Characterizations of prepared LPPC revealed that the maximal-binding capacity was about 170 mu g of bovine serum albumin to 40 mu g of sphere-shaped LPPC (180 nm). The proteins were essentially located at or near the surface when analyzed by atomic force or transmission electron microscopy. We demonstrate that polyethylenimine was an essential component to bind the proteins. Upon the saturation of captured proteins, a given protein could not be displaced by other additional proteins and still retained its biological activity. Using a variety of functional proteins, we show some typical examples of the utility of incorporated beta-glucuronidase and antibodies onto the LPPC. The beta-glucuronidase can be used for the study of antigen-antibody interactions, whereas in studies with the antibody complex, we used anti-CD3 as an agonist to stimulate the proliferation of peripheral blood mononuclear cells via a receptor-mediated mechanism and anti-VEGFR for cell staining. In conclusion, the prepared LPPC can provide a platform to capture biologically and biochemically functional proteins on its surface for various applications, such as cell signaling, cell profiling, noncovalent enzyme-linked immunoassays, and others not mentioned. Biotechnol. Bioeng. Biotechnol. Bioeng. 2011; 108: 1318-1327. (C) 2010 Wiley Periodicals, Inc.
URI: http://dx.doi.org/10.1002/bit.23048
http://hdl.handle.net/11536/8824
ISSN: 0006-3592
DOI: 10.1002/bit.23048
期刊: BIOTECHNOLOGY AND BIOENGINEERING
Volume: 108
Issue: 6
起始頁: 1318
結束頁: 1327
顯示於類別:期刊論文


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