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dc.contributor.authorTzeng, Yu-Pingen_US
dc.contributor.authorShen, Ching-Weien_US
dc.contributor.authorYu, Tiingen_US
dc.date.accessioned2014-12-08T15:11:22Z-
dc.date.available2014-12-08T15:11:22Z-
dc.date.issued2008-06-06en_US
dc.identifier.issn0021-9673en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.chroma.2008.02.118en_US
dc.identifier.urihttp://hdl.handle.net/11536/8727-
dc.description.abstractAn affinity-dye, Cibacron Blue 3GA (CB), derivatized organic salt [BMIM](3)[CB] was synthesized for lysozyme extraction. This compound was formed by mixing an ionic liquid (IL) [BMIM][CI] and the silver salt of CB. Liquid-liquid extractions of lysozyme from the aqueous and [BMIM](3)[CB] in [BMIM][PF6] solutions were examined in this study. The transfer of lysozyme from the aqueous phase to the IL phase decreased while the pH of the aqueous phase increased. An extraction higher than 90% was observed at pH 4. Under a high ionic strength, the lysozyme would transform back from the IL phase into the aqueous phase. Lysozyme molecules were almost quantitatively recovered from the IL phase to the aqueous solutions of 1 M KCl under pH 9-11. It appeared that the extraction was specific for lysozyme in contrast to cytochrome c, ovalbumin, and bovine serum albumin. The extraction efficiency of the IL phase remained essentially the same after eight cycles of extraction. (c) 2008 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USen_US
dc.subjectionic liquiden_US
dc.subjectaffinity extractionen_US
dc.subjectprotein purificationen_US
dc.subjectCibacron Blue 3GAen_US
dc.subjectlysozymeen_US
dc.titleLiquid-liquid extraction of lysozyme using a dye-modified ionic liquiden_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.chroma.2008.02.118en_US
dc.identifier.journalJOURNAL OF CHROMATOGRAPHY Aen_US
dc.citation.volume1193en_US
dc.citation.issue1-2en_US
dc.citation.spage1en_US
dc.citation.epage6en_US
dc.contributor.department應用化學系zh_TW
dc.contributor.departmentDepartment of Applied Chemistryen_US
dc.identifier.wosnumberWOS:000256558600001-
dc.citation.woscount21-
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