標題: Protein plasticity: A single amino acid substitution in the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase generates protosta-13(17),24-dien-3 beta-ol, a rearrangement product
作者: Wu, Tung-Kung
Wen, Hao-Yu
Chang, Cheng-Hsiang
Liu, Yuan-Ting
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
公開日期: 19-Jun-2008
摘要: To provide insights into the structure-function relationships of oxidosqualene-lanosterol cyclase (ERG7) from Saccharomyces cerevisiae, the Phe699 was exchanged against hydrophilic polar uncharged residues Ser, Thr, Cys, Gin, and Tyr to characterize its product profile and functional role in ERG7 activity. Among the substitutions, only the ERG7(F699T) mutant produced novel protosta-13(17),24-dien-3 beta-ol as the sole truncated rearrangement product. The results suggest that Phe699Thr mutation is likely to affect the C-17 cation stabilization during the rearrangement process.
URI: http://dx.doi.org/10.1021/ol800799n
http://hdl.handle.net/11536/8711
ISSN: 1523-7060
DOI: 10.1021/ol800799n
期刊: ORGANIC LETTERS
Volume: 10
Issue: 12
起始頁: 2529
結束頁: 2532
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