標題: A possibility of detection of the non-charge based analytes using ultra-thin body field-effect transistors
作者: Sheu, J. -T.
Chen, C. C.
Chang, K. S.
Li, Y. -K.
材料科學與工程學系奈米科技碩博班
應用化學系
Graduate Program of Nanotechnology , Department of Materials Science and Engineering
Department of Applied Chemistry
關鍵字: biosensors;ultra-thin body field-effect transistor;N-[3(trimethoxysilyl)propyl]ethylenediamine;gold nanoparticles;Delta(5)-3-ketosteroid isomerase;19-norandrostendione
公開日期: 15-七月-2008
摘要: Ultra-thin body of p-type field-effect transistors were developed as transducer for biosensors. Changes of conductance resulted from the changes of the surface potentials of ultra-thin body field-effect transistors (UTB-FETs) due to surface chemical modifications were demonstrated. The channel surface of UTB-FETs were modified with N-[3-(trimethoxysilyl)propyl]ethylenediamine (AEAPTMS) and then gold nanoparticles (AuNPs) to immobilize the bio-component, the genetically engineered Delta(5)-3-ketosteroid isomerase (Art_KSI) or the Art_KSI conjugated with charged reporter (Art_KSI_mA51). The binding of charge-based molecules or nanoparticles has been demonstrated to strongly affect the conductivity of UTB-FETs: the increase or decrease of the conductance depends on the polarity of the immobilized molecules or nanoparticles. A new protocol involving the detection of a non-charged analyte relied on the competitive binding of analyte (19-norandrostendione) and a charged reporter (mA51) with KSL When exposed to a 19-norandrostendione solution (10 mu M), the conductance of Art_KSI_mA51-modified UTB-FET increased by 265 nS (similar to 12%). On the other hand, conductance of Art_KSI_modified UTB-FET showed no distinct change under the same detection conditions. (c) 2008 Elsevier B.V. All rights reserved
URI: http://dx.doi.org/10.1016/j.bios.2008.02.024
http://hdl.handle.net/11536/8576
ISSN: 0956-5663
DOI: 10.1016/j.bios.2008.02.024
期刊: BIOSENSORS & BIOELECTRONICS
Volume: 23
Issue: 12
起始頁: 1883
結束頁: 1886
顯示於類別:期刊論文


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