標題: Cloning and characterization of monacolin K biosynthetic gene cluster from Monascus pilosus
作者: Chen, Yi-Pei
Tseng, Ching-Ping
Liaw, Li-Ling
Wang, Chun-Lin
Chen, I-Ching
Wu, Wen-Jung
Wu, Ming-Der
Yuan, Gwo-Fang
生物科技學系
Department of Biological Science and Technology
關鍵字: monacolin K;polyketide synthases;Monascus pilosus;bacterial artificial chromosome
公開日期: 23-七月-2008
摘要: Monacolin K is a secondary metabolite synthesized by polyketide synthases (PKS) from Monascus, and it has the same structure as lovastatin, which is mainly produced by Aspergillus terreus. In the present study, a bacterial artificial chromosome (BAC) clone, mps01, was screened from the BAC library constructed from Monascus pilosus BCRC38072 genomic DNA. The putative monacolin K biosynthetic gene cluster was found within a 42 kb region in the mps01 clone. The deduced amino acid sequences encoded by the nine genes designated as mokA-mokl, which share over 54% similarity with the lovastatin biosynthetic gene cluster in A. terreus, were assumed to be involved in monacolin K biosynthesis. A gene disruption construct designed to replace the central part of mokA, a polyketide synthase gene, in wild-type M. pilosus BCRC38072 with a hygromycin B resistance gene through homologous recombination, resulted in a mokA-disrupted strain. The disruptant did not produce monacolin K, indicating that mokA encoded the PKS responsible for monacolin K biosynthesis in M. pilosus BCRC38072.
URI: http://dx.doi.org/10.1021/jf800595k
http://hdl.handle.net/11536/8558
ISSN: 0021-8561
DOI: 10.1021/jf800595k
期刊: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume: 56
Issue: 14
起始頁: 5639
結束頁: 5646
顯示於類別:期刊論文


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  1. 000257721400029.pdf