標題: Specific DNA extraction through fluid channels with immobilization of layered double hydroxides on polycarbonate surface
作者: Chan, Chia-Hao
Chen, Jem-Kun
Chang, Feng-Chih
應用化學系
Department of Applied Chemistry
關鍵字: layered double hydroxides;lab-on-a-chip;nano-extraction;fluidic system
公開日期: 28-七月-2008
摘要: The purpose of this study was to immobilize inorganic layered double hydroxides (LDHs) on the polycarbonate (PC) substrate as the media to extract the specific DNA molecules through fluidic system and to enhance the extraction efficiency of specific DNA molecules from extreme low concentration in sample solution. LDH immobilized through solvent swelling and plasma treatment on the polymer surface captured the specific DNA molecules lysed from Escherichia coli (E. call) cells as the target DNA molecules with 2 x 10(-4) g/l of concentration in sample solution mixed biomacromolecules lysed from human blood. The encapsulated DNA molecules released through dissolve of LDHs by slight acid (pH 4-5) solution then amplified by polymerase chain reaction (PCR) process through the primers for E coli cells. The DNA molecules amplified by PCR process were characterized by gel electrophoresis to recognize the existence of E. coli cells. The results show that immobilized LDHs could be regarded as the specific DNA detector in the sample solution mixed biomacromolecules for rapid disease diagnosis through fluidic system to approach the lab-on-a-chip (LOC). (c) 2008 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.snb.2008.02.041
http://hdl.handle.net/11536/8553
ISSN: 0925-4005
DOI: 10.1016/j.snb.2008.02.041
期刊: SENSORS AND ACTUATORS B-CHEMICAL
Volume: 133
Issue: 1
起始頁: 327
結束頁: 332
顯示於類別:期刊論文


文件中的檔案:

  1. 000258011000048.pdf