標題: Peak crossover in high-performance liquid chromatography elution monitored using whole-column detection
作者: Lin, Shu-Hui
Yu, Tiing
Sheu, Alf
Yang, Der-Jyh
Pai, Su-Cheng
應用化學系
Department of Applied Chemistry
關鍵字: peak crossover;retention-order inversion;whole-column detection;multi-channel detection;HPLC
公開日期: 1-八月-2008
摘要: A whole-column detection (WCD) system was fabricated using the parts from a typical A4 size optical scanner. Optimum layout of the fluorescence tube, beam condenser and photon transducers from the scanner made an effective devise to examine the dynamic chromatographic separation process. This system was applied in monitoring peak crossover in an ion-pair chromatographic elution. A stage-wise gradient was conducted in eluting a mixture of New Coccine and Carmoisine. Compound New Coccine lagging behind after sample injection moved passing Carmoisine at the column outlet. The complete on-column elution process could be observed directly using this detection system. This inexpensive and rugged WCD system provided a 0.3 mm spatial resolution and a 3.6 ms temporal resolution and was proven to be an adequate device to directly monitor solute retention behavior in the liquid chromatography column. (c) 2008 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.chroma.2008.06.018
http://hdl.handle.net/11536/8535
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2008.06.018
期刊: JOURNAL OF CHROMATOGRAPHY A
Volume: 1201
Issue: 1
起始頁: 128
結束頁: 131
顯示於類別:期刊論文


文件中的檔案:

  1. 000258112300018.pdf