|標題:||Selection of an Effective Red-Pigment Producing Monascus pilosus by Efficient Transformation with Aurintricarboxylic Acid|
Department of Biological Science and Technology
|關鍵字:||Monascus pilosus;restriction-enzyme-mediated integration (REMI);aurintricarboxylic acid (ATA)|
|摘要:||The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation.|
|期刊:||BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY|
|Appears in Collections:||Articles|
Files in This Item:
If it is a zip file, please download the file and unzip it, then open index.html in a browser to view the full text content.