標題: Selection of an Effective Red-Pigment Producing Monascus pilosus by Efficient Transformation with Aurintricarboxylic Acid
作者: Chen, Yi-Pei
Chen, I-Ching
Hwang, Ing-Er
Yuan, Gwo-Fang
Liaw, Li-Ling
Tseng, Ching-Ping
生物科技學系
Department of Biological Science and Technology
關鍵字: Monascus pilosus;restriction-enzyme-mediated integration (REMI);aurintricarboxylic acid (ATA)
公開日期: 1-Nov-2008
摘要: The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation.
URI: http://dx.doi.org/10.1271/bbb.80342
http://hdl.handle.net/11536/8162
ISSN: 0916-8451
DOI: 10.1271/bbb.80342
期刊: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume: 72
Issue: 11
起始頁: 3021
結束頁: 3024
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