Molecular Phylogenetic Relationships of Cecidomyiidae Gall Midges and Proteomics of Galls from Machilus Hosts
|關鍵字:||蟲癭;癭蚋科;楨楠;演化;蛋白質體;gall midges;cecidomyiidae;machilus;DNA barcode;phylogeny;proteomics|
Cecidology is the study of plant galls, where insects and other organisms breed their offsprings. Cecidology crosses multiple areas such as ecology, insect taxonomy, botanical taxonomy, botanic pathology, parasitology, and it’s a special research subject. Little is known regarding the interaction between gall insects and their host at molecular level. In Taiwan, Machilus (Lauraceae) plants have the widest variety of gall shapes among all plant species. Cecidomyiidae (Dipteria) is the major gall inducing insects on Machilus. To understand the relationship between gall insects and their hosts, we first identified different species of gall insect through insect genomics and the responses of the host were studied through plant proteomics. In insect genomics, we used COI gene to classify 40 unknown species collected from 9 different types of galls on 8 Machilus species. These cecidomyiidae gall inducing midges could be classified into 7 tribes, 11 genus, and 15 species. Our data indicate that insects which make same type of galls are closer species. The anatomical evidence and ecological theories also supports genomic evidence. In plant proteomics, we found that galls were benignancy tissue growth of plant. Because RuBisCO protein expression in gall tissues was largely decreased, galls might be the storage organisms of plants. Combine genomic and proteomic outcomes, we suggest that same or closer insect species secretes same chemicals to induce same shape of galls, and it has nothing to do with host plants within Machilus genus. Some proteins might also affect the forming of different gall shapes as significant protein variations were observed between gall and normal plant tissues.
|Appears in Collections:||Thesis|
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