Title: Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7
Authors: Liu, Yi-Ju
Yao, Da-Jeng
Chang, Hwan-You
Liu, Chien-Ming
Chen, Chih
Department of Materials Science and Engineering
Keywords: DNA detection;Magnetic bead;Quantum dots;Biotin-streptavidin;Biotinlyted linker
Issue Date: 1-Dec-2008
Abstract: This work demonstrated the feasibility of detecting 250 zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60 min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis. (C) 2008 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.bios.2008.06.019
ISSN: 0956-5663
DOI: 10.1016/j.bios.2008.06.019
Volume: 24
Issue: 4
Begin Page: 558
End Page: 565
Appears in Collections:Articles

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