標題: Determination of saikosaponins by micellar electrokinetic capillary chromatography
作者: Hsieh, YZ
Huang, HY
交大名義發表
應用化學系
National Chiao Tung University
Department of Applied Chemistry
關鍵字: buffer composition;pharmaceutical analysis;saikosaponins;saponins
公開日期: 24-一月-1997
摘要: In this study, we examine the feasibility of determining saikosaponins a, b1, b2, c and d, i.e., the bioactive components in Bupleuri Radix, using the micellar electrokinetic capillary chromatography technique. Results obtained from using different anion surfactants, bile salt or sodium dodecyl sulfate (SDS), are also compared. By using bile salt as a pseudostationary phase, the baseline separation of saikosaponins is observed; however, the detection limit is unfavorable. Whereas the baseline resolution of saikosaponins is achieved after adding gamma-cyclodextrin in the SDS running buffer. In addition, another separating method is developed with the mixed micellar system of SDS and Brij 35 containing gamma-cyclodextrin. The contents of saikosaponins in traditional Chinese medicinal prescriptions, such as Sheau Chair Hwu Tang, Bupleuri Radix plant and Chair Hwu San are analyzed by one of the above methods. The entire separation can be completed within 9 min by these methods. The R.S.D. values of the migration times are all below 1%. Moreover, the detection limits of saikosaponins vary from 7 to 33 mu g/ml.
URI: http://dx.doi.org/10.1016/S0021-9673(96)00768-6
http://hdl.handle.net/11536/771
ISSN: 0021-9673
DOI: 10.1016/S0021-9673(96)00768-6
期刊: JOURNAL OF CHROMATOGRAPHY A
Volume: 759
Issue: 1-2
起始頁: 193
結束頁: 201
顯示於類別:期刊論文


文件中的檔案:

  1. A1997WH31200019.pdf