Title: Quantitative Assessment of Hepatic Fat of Intact Liver Tissues with Coherent Anti-Stokes Raman Scattering Microscopy
Authors: Wu, Yao-Ming
Chen, Hung-Che
Chang, Wei-Tien
Jhan, Jhen-Wei
Lin, Hung-Lung
Liau, Ian
Department of Applied Chemistry
Institute of Molecular science
Issue Date: 15-Feb-2009
Abstract: A fatty liver might progress from being a benign fatty liver, to steatohepatitis, cirrhosis, or even hepatocellular carcinoma. The great prevalence and severe outcome have warranted much investigation of the pathology and the development of effective therapies, which involve animal studies requiring critical evaluation of the hepatic fatty change. Histological examination and wet chemical analysis of liver biopsy specimens are generally employed for this purpose despite numerous procedures being involved. Using coherent anti-Stokes Raman scattering (CARS) microscopy, we have demonstrated the specific imaging of fat droplets in intact liver tissues and extracted the hepatic fat content through image analysis while eliminating laborious procedures required by traditional histopathological. examination. The content of hepatic fat measured with CARS imaging was correlated strongly with that determined by biochemical analysis (R(2) = 0.89) over a pathologically significant range of the hepatic fat (from 2% to 20% of the total mass of tissue). Our work validates the quantitative assessment of fat in intact tissue through the use of CARS microscopy. When combined with the increasingly diverse animal models of diseases related to metabolic disorders of lipids, our approach is extensible to enable acquiring important insight into the genetic, environmental, and dietary factors affecting the uptake and accumulation of fat within tissues.
URI: http://dx.doi.org/10.1021/ac8026838
ISSN: 0003-2700
DOI: 10.1021/ac8026838
Volume: 81
Issue: 4
Begin Page: 1496
End Page: 1504
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