標題: Quantitative Assessment of Hepatic Fat of Intact Liver Tissues with Coherent Anti-Stokes Raman Scattering Microscopy
作者: Wu, Yao-Ming
Chen, Hung-Che
Chang, Wei-Tien
Jhan, Jhen-Wei
Lin, Hung-Lung
Liau, Ian
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
公開日期: 15-二月-2009
摘要: A fatty liver might progress from being a benign fatty liver, to steatohepatitis, cirrhosis, or even hepatocellular carcinoma. The great prevalence and severe outcome have warranted much investigation of the pathology and the development of effective therapies, which involve animal studies requiring critical evaluation of the hepatic fatty change. Histological examination and wet chemical analysis of liver biopsy specimens are generally employed for this purpose despite numerous procedures being involved. Using coherent anti-Stokes Raman scattering (CARS) microscopy, we have demonstrated the specific imaging of fat droplets in intact liver tissues and extracted the hepatic fat content through image analysis while eliminating laborious procedures required by traditional histopathological. examination. The content of hepatic fat measured with CARS imaging was correlated strongly with that determined by biochemical analysis (R(2) = 0.89) over a pathologically significant range of the hepatic fat (from 2% to 20% of the total mass of tissue). Our work validates the quantitative assessment of fat in intact tissue through the use of CARS microscopy. When combined with the increasingly diverse animal models of diseases related to metabolic disorders of lipids, our approach is extensible to enable acquiring important insight into the genetic, environmental, and dietary factors affecting the uptake and accumulation of fat within tissues.
URI: http://dx.doi.org/10.1021/ac8026838
http://hdl.handle.net/11536/7624
ISSN: 0003-2700
DOI: 10.1021/ac8026838
期刊: ANALYTICAL CHEMISTRY
Volume: 81
Issue: 4
起始頁: 1496
結束頁: 1504
顯示於類別:期刊論文


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