標題: 開發高深寬比微型化高分子層析管柱應用於高效能液相層析儀器
Development of High-aspect-ratio Miniaturization Polymer Column for High Performance Liquid Chromatography
作者: 張貴棠
Chang, Kuei-Tang
徐文祥
Hsu, Wensyang
機械工程系所
關鍵字: 層析;Column
公開日期: 2014
摘要: 層析管柱在目前分離技術(電泳法、液相層析法、高效能液相層析法)中,是個不可或缺的部分。層析管柱主要是功能是分離(純化),其原理是利用分離樣品親和力的不同,而產生滯留時間的差異進行分離,其結構及材料都會影響分離效果。 在目前製作微型化的層析管柱文獻中,大多數設計均為圓形柱狀陣列的結構,且在材料上大多數以矽、高分子兩種為主。矽基層析管柱是大多數學者研究的方向,目前深寬比能製作到高達40且運用在高效能層析儀進行分離測試。高分子層析管柱的特點是能使用翻印技術快速製成,但常遇到楊氏係數不足的問題,容易頃倒,且目前高分子深寬比已達18。本次研究重心放在製成高深寬比高分子層析管柱且測試其分離效能,希望能將高分子層析管柱的深寬比提高到與矽基層析管柱接近,且能有效的縮短分離時間,達到快速檢測的目的。 本研究將製成矽基層析管柱以及高分子層析管柱並與商用層析管柱作分離效能的比較。從結果得知,可以成功製作出深寬比約為32的PDMS層析管柱以及深寬比約為31的矽基層析管柱,且兩種管柱都成功分離出苯與二苯甲酮兩種樣品。矽基層析管柱與高分子層析管柱分離時間皆僅需約1.5分鐘,均優於商用層析管柱分離時間。
Column is an important part in the separation techniques (Electrophoresis, Liquid Chromatography, High Performance Liquid Chromatography) in recently. Main function of the Column is to separate (purified) the sample. The principle is make use of different affinity of sample cause to the different retard time to separation. Its structure and material will affect the separation efficiency. The most literature of miniaturized column is designed to the circular pillar array in the current, and the majority of the material is silicon-based or polymer-based. Silicon-based column is the major direction of the research. The aspect ratio can be up to 40 and apply to high-performance liquid chromatography. The characterized of Polymer-based column can be made quickly by the reprographic technology, but often encounter the problem of inadequate Young's modulus will be fall. The research focus to make of high aspect ratio polymer-based column and test its separation efficiency. Hoping the aspect ratio of polymer column can be made as the same as silicon-based column, and shorten the separation time to achieve the purpose of rapid detection. This study will compare separation sufficiency with silicon-based, polymer-based and commercial column. From the results, this experiment can successfully create a PDMS column aspect ratio about 32, and silicon column aspect ratio about 31.Both column are successfully separate the sample of benzene and benzophenone. Silicon polymer The separation time is only about 1.5 minutes for Silicon-based and polymer-based column. The separation time of commercial column is less than above.
URI: http://140.113.39.130/cdrfb3/record/nctu/#GT070151028
http://hdl.handle.net/11536/76120
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