Partial Pressure Estimation of Brain Tissue Oxygen in Traumatic Brain Injury by Using Wireless Near Infrared Spectroscopy
|關鍵字:||腦創傷;近紅外線光譜儀;含氧血紅素;非含氧血紅素;全血紅素;2,3,5氯化三苯基四氮唑;腦組織氧分壓;traumatic brain injury;near-infrared spectroscopy;oxyhemoglobin;deoxyhemoglobin;totalhemoglobin;triphenyltetrazolium chloride (TTC);partial pressure of oxygen|
Monitoring partial pressure of oxygen in the brain tissue (PbtO2) is an important standard for traumatic brain injury (TBI) patients in clinical. But it is an invasive measurement and inconvenient for real-time monitoring. Recently, Near-infrared spectroscopy (NIRS) is widely used in neuroscience, and can assess cerebral ischemia and hypoxia non-invasively. In this thesis, a novel wireless NIRS system, and the PbtO2 monitoring system were used to monitor the oxygenation of rat brains under different impact strengths. And all rats were randomly assigned to four different impact strength groups in the fluid percussion injury experiments. The relationships of the concentration changes of HbO2 and HbR, and PbtO2 under and after TBI with different impact strengths were also investigated. Triphenyltetrazolium chloride (TTC) staining was used for infarction volume evaluation. Results show that Δ[HbO2], Δ[HbR], and Δ[HbT] dropped immediately after the impact and increased gradually then maintain a stable status. And Δ[PbtO2] had a similar change tendency with the NIRS parameters. The result of the TTC staining showed the infarction volume was increased with the increased impact strength, and Δ[HbO2] and Δ[PbtO2] were decreased with the increased impact strength. The correlation coefficient between Δ[PbtO2] and Δ[HbO2] is 0.77, and between Δ[HbR] and Δ[HbO2] is 0.08. It can be discovered that Δ[HbO2] is highly and positively correlated to Δ[PbtO2]. That means the Δ[HbO2] may be available to be the reference parameter to estimate the partial pressure of oxygen in the brain tissue.