Title: Iron Oxide/Niobium Oxide Core-Shell Magnetic Nanoparticle-Based Phosphopeptide Enrichment from Biological Samples for MALDI MS Analysis
Authors: Lin, Hong-Yi
Chen, Wei-Yu
Chen, Yu-Chie
Department of Applied Chemistry
Keywords: Magnetic Nanoparticles;Phosphopeptides;MALDI MS;Niobium Oxide;Microwave-Heating
Issue Date: 1-Apr-2009
Abstract: The feasibility of using niobium oxide-coated magnetic nanoparticles (NPs) as affinity probes for selectively trapping phosphopeptides from peptide mixtures including tryptic digest of caseins, serum, and cell lysate was demonstrated in this study. Phosphopeptide enrichment was rapid when subjecting these systems to microwave heating for 1 min; the probe-target species, which were readily isolated through magnetic separation, were then analyzed using matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Only signals for phosphopeptides were present in the resulting mass spectra. The detection limit for monophosphopeptide was as low as 5 fmol.
URI: http://dx.doi.org/10.1166/jbn.2009.1022
ISSN: 1550-7033
DOI: 10.1166/jbn.2009.1022
Volume: 5
Issue: 2
Begin Page: 215
End Page: 223
Appears in Collections:Articles