標題: Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from Candida tropicalis
作者: Chen, Li-Chun
Huang, Sheng-Cih
Chuankhayan, Phimonphan
Chen, Chung-Der
Huang, Yen-Chieh
Jeyakanthan, Jeyaraman
Pang, Hsiao-Fang
Men, Lee-Chung
Chen, Yu-Ching
Wang, Yu-Kuo
Liu, Ming-Yih
Wu, Tung-Kung
Chen, Chun-Jung
生物科技學系
Department of Biological Science and Technology
公開日期: 1-Apr-2009
摘要: Xylose reductase (XR), which requires NADPH as a co-substrate, catalyzes the reduction of D-xylose to xylitol, which is the first step in the metabolism of D-xylose. The detailed three-dimensional structure of XR will provide a better understanding of the biological significance of XR in the efficient production of xylitol from biomass. XR of molecular mass 36.6 kDa from Candida tropicalis was crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction data from C. tropicalis XR crystals at 2.91 angstrom resolution, the unit cell belongs to space group P3(1) or P3(2). Preliminary analysis indicated the presence of four XR molecules in the asymmetric unit, with 68.0% solvent content.
URI: http://dx.doi.org/10.1107/S1744309109008719
http://hdl.handle.net/11536/7393
ISSN: 1744-3091
DOI: 10.1107/S1744309109008719
期刊: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
Volume: 65
Issue: 
起始頁: 419
結束頁: 421
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