標題: Surface Reaction Limited Model for the Evaluation of Immobilized Enzyme on Planar Surfaces
作者: Lee, Cheng-Che
Chiang, Han-Ping
Li, Kun-Lin
Ko, Fu-Hsiang
Su, Chien-Ying
Yang, Yuh-Shyong
材料科學與工程學系奈米科技碩博班
生物科技學系
Graduate Program of Nanotechnology , Department of Materials Science and Engineering
Department of Biological Science and Technology
公開日期: 1-Apr-2009
摘要: Analysis of immobilized enzyme in situ is a crucial step to embed an enzyme onto the planar technology of standard integrated circuit (IC) and microelectromechanical systems (MEMS) for a bioreactor or enzyme-coupled biosensor. A surface reaction limited model, based on a systematized and standardized approach, mathematically derived from mass transfer dynamics and the Michaelis-Menten equation for the measuring the apparent K(m)(star) (Michaelis-Menten constant) and V(max)(star) (maximum reaction rate per unit surface area of catalyst) of an immobilized enzyme on a planar surface was developed. The derived equations for the kinetic model were simulated and experimentally confirmed. A platform of a microflow bioreactor with a one-sided planar catalytic surface that contained immobilized enzyme was constructed. The microfluidic bioreactor was designed to possess a channel height less than that of the diffusion layer thickness in a semi-infinite diffusion process, and K(m)(star) and V(max)(star) of rat phenol sulfotransferase (PST) immobilized on the silicon oxide surface were successfully determined in situ. Variation in kinetic constants and the possible differences in performance between free and immobilized PST are discussed.
URI: http://dx.doi.org/10.1021/ac802650k
http://hdl.handle.net/11536/7386
ISSN: 0003-2700
DOI: 10.1021/ac802650k
期刊: ANALYTICAL CHEMISTRY
Volume: 81
Issue: 7
起始頁: 2737
結束頁: 2744
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