標題: Neutrophils as one of the major haptoglobin sources in mastitis affected milk
作者: Lai, I-Hsiang
Tsao, Jung Hsu
Lu, Yi Ping
Lee, Jai Wei
Zhao, Xin
Chien, Feng Lin
Mao, Simon J. T.
生物科技學系
Department of Biological Science and Technology
關鍵字: haptoglobin;neutrophil;mastitis;MAC-T cells;immunocytochemistry
公開日期: 1-May-2009
摘要: The antioxidant haptoglobin (Hp) is an acute-phase protein responsive to infectious and inflammatory diseases. Hp and somatic cell counts (SCC) are sharply elevated in bovine milk following intramammary administration of endotoxin or bacteria. However, the sources of milk Hp responsible for such increases are not fully understood. The purpose of this study was to de. ne the source of milk Hp from dairy cows with naturally occurring mastitis. Quarter milk samples selected from 50 dairy cows were separated into four groups according to SCC as group A: < 100 (n = 19); B: 100-200 (n = 10); C: 201-500 (n = 10); and D: > 500 x 10(3) (n = 11) cells/mL. Our results reveal that milk Hp concentrations were correlated with SCC (r = 0.742; P < 0.01), and concentrations in group D were similar to 10-fold higher than in group A. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis indicates that the milk somatic cells from group D were not only capable of synthesizing Hp but could also markedly increase Hp mRNA expression. Western blot, immunocytochemistry, double confocal immuno fluorescence, and Hp releasing experiments demonstrate that neutrophils were associated with the biosynthesis and release of Hp in milk. It further shows that Hp was significantly elevated in the epithelium of mammary gland tissue with mastitis and was also expressed in the cultured mammary epithelial cells. We propose that neutrophils and epithelial cells may play an essential role in elevating milk Hp in addition to previous suggestions that Hp may be derived from mammary tissues and circulation.
URI: http://dx.doi.org/10.1051/vetres:2008055
http://hdl.handle.net/11536/7276
ISSN: 0928-4249
DOI: 10.1051/vetres:2008055
期刊: VETERINARY RESEARCH
Volume: 40
Issue: 3
結束頁: 
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