標題: Separation of retinoids by micellar electrokinetic capillary chromatography
作者: Hsieh, YZ
Kuo, KL
交大名義發表
應用化學系
National Chiao Tung University
Department of Applied Chemistry
關鍵字: retinoids;vitamin A
公開日期: 14-二月-1997
摘要: In this study, we have developed a micellar electrokinetic capillary chromatography (MEKC) method with diode-array detection to separate five important retinoids, including retinol, retinal, retinoic acid, retinyl acetate and retinyl palmitate. These very hydrophobic retinoids are successfully separated with phosphate buffer containing sodium deoxycholate (SDC) and Brij 35 as the micellar pseudostationary phase. The effects of SDC and Brij 35 concentration on the migration behavior of the five retinoids are also discussed. The relative standard deviations of the migration times of the retinoids are less than 1.9% in the optimized separation condition. Moreover, a direct extraction method without saponification is employed to extract retinyl esters in pharmaceutical products. The ranges of recoveries are 95%-99% for retinyl esters with less than 2% relative standard deviations. Therefore, retinyl esters in pharmaceutical products can be determined by combining the direct extraction and the MEKC methods.
URI: http://dx.doi.org/10.1016/S0021-9673(96)00820-5
http://hdl.handle.net/11536/725
ISSN: 0021-9673
DOI: 10.1016/S0021-9673(96)00820-5
期刊: JOURNAL OF CHROMATOGRAPHY A
Volume: 761
Issue: 1-2
起始頁: 307
結束頁: 313
顯示於類別:期刊論文


文件中的檔案:

  1. A1997WM84600030.pdf