標題: Micropatterning of perfluoroalkyl self-assembled monolayers for arraying proteins and cells on chips
作者: Kira, Atsushi
Okano, Kazunori
Hosokawa, Yoichiroh
Naito, Akira
Fuwa, Koh
Yuyama, Jyunpei
Masuhara, Hiroshi
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: Chemical surface modification;Two-dimensional patterning of self-assemble monolayer;Protein patterning;Cell patterning;Microarray;Microfabrication
公開日期: 15-Jun-2009
摘要: Organosilane self-assembled monolayers (SAMs) with perfluoroalkyl groups (R(f)) on glass surfaces were used for arraying proteins and cells on chips. Quartz crystal microbalance measurements confirmed the inhibition of protein adsorption on R(f)-SAM-modified surfaces and showed efficient adsorption on hydroxyl-, carboxyl-, and amino group-modified surfaces. The characteristics of R(f)-modified surfaces were evaluated using solvent contact angle measurement and Fourier transform infrared (FTIR) spectroscopy. The R(f) surface was highly water-and oil-resistant, as inferred from the contact angles of water, oleic acid, and hexadecane. Specific peaks of IR spectra were detected in the region from 1160 to 1360 cm(-1). Etching with dry plasma completely exfoliated the R(f)-SAM, exposing the underlying intact glass surface. Modi. cation conditions were optimized using contact angle and FTIR measurements. After dry plasma processing, the contact angles of all solvents became undetectable, and the IR peaks disappeared. Micrometer scale protein and cell patterns can be fabricated using the proposed method. Protein adsorption on micropatterned R(f)-SAM-modified chips was evaluated using fluorescence analysis; protein adsorption was easily controlled by patterning R(f)-SAM. PC12 and HeLa cells grew well on micropatterned R(f)-SAM-modified chips. Micropatterning of R(f)-SAM by dry plasma treatment with photolithography is useful for the spatial arrangement of proteins and cells. (C) 2009 Elsevier B. V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.apsusc.2009.04.044
http://hdl.handle.net/11536/7110
ISSN: 0169-4332
DOI: 10.1016/j.apsusc.2009.04.044
期刊: APPLIED SURFACE SCIENCE
Volume: 255
Issue: 17
起始頁: 7647
結束頁: 7651
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