標題: Isolation and characterisation of a novel angiotensin I-converting enzyme (ACE) inhibitory peptide from the algae protein waste
作者: Sheih, I-Chuan
Fang, Tony J.
Wu, Tung-Kung
生物科技學系
Department of Biological Science and Technology
關鍵字: Microalgae;Chlorella vulgaris;Hypertension;Angiotensin 1-converting enzyme
公開日期: 1-Jul-2009
摘要: A hendeca-peptide with angiotensin I-converting enzyme (ACE) inhibitory activity was isolated from the pepsin hydrolysate of algae protein waste, a mass-produced industrial by-product of an algae essence from microalgae, Chlorella vulgaris. Edman degradation revealed its amino acid sequence to be Val-Glu-Cys-Tyr-Gly-Pro-Asn-Ai-g-Pro-Gln-Phe. Inhibitory kinetics revealed a non-competitive binding made with IC(50) Value against ACE of 29.6 mu M, suggesting a potent amount of ACE inhibitory activity compared with other peptides from the microalgae protein hydrolysates which have a reported range between 11.4 and 315.3 mu M. In addition, the purified hendeca-peptide completely retained its ACE inhibitory activity at a pH range of 2-10, temperatures of 40-100 degrees C, as well as after treatments in vitro by a gastrointestinal enzyme, thus indicating its heat- and pH-stability. The combination of the biochemical properties of this isolated hendeca-peptide and a cheap algae protein resource make an attractive alternative for producing a high value product for blood pressure regulation as well as water and fluid balance. (C) 2008 Elsevier Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.foodchem.2008.12.019
http://hdl.handle.net/11536/7061
ISSN: 0308-8146
DOI: 10.1016/j.foodchem.2008.12.019
期刊: FOOD CHEMISTRY
Volume: 115
Issue: 1
起始頁: 279
結束頁: 284
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