標題: Computational identification of riboswitches based on RNA conserved functional sequences and conformations
作者: Chang, Tzu-Hao
Huang, Hsien-Da
Wu, Li-Ching
Yeh, Chi-Ta
Liu, Baw-Jhiune
Horng, Jorng-Tzong
生物資訊及系統生物研究所
Institude of Bioinformatics and Systems Biology
關鍵字: riboswitch;RNA secondary structure;regulatory RNA
公開日期: 1-七月-2009
摘要: Riboswitches are cis-acting genetic regulatory elements within a specific mRNA that can regulate both transcription and translation by interacting with their corresponding metabolites. Recently, an increasing number of riboswitches have been identified in different species and investigated for their roles in regulatory functions. Both the sequence contexts and structural conformations are important characteristics of riboswitches. None of the previously developed tools, such as covariance models (CMs), Riboswitch finder, and RibEx, provide a web server for efficiently searching homologous instances of known riboswitches or considers two crucial characteristics of each riboswitch, such as the structural conformations and sequence contexts of functional regions. Therefore, we developed a systematic method for identifying 12 kinds of riboswitches. The method is implemented and provided as a web server, RiboSW, to efficiently and conveniently identify riboswitches within messenger RNA sequences. The predictive accuracy of the proposed method is comparable with other previous tools. The efficiency of the proposed method for identifying riboswitches was improved in order to achieve a reasonable computational time required for the prediction, which makes it possible to have an accurate and convenient web server for biologists to obtain the results of their analysis of a given mRNA sequence. RiboSW is now available on the web at http://RiboSW.mbc.nctu.edu.tw/.
URI: http://dx.doi.org/10.1261/rna.1623809
http://hdl.handle.net/11536/7060
ISSN: 1355-8382
DOI: 10.1261/rna.1623809
期刊: RNA-A PUBLICATION OF THE RNA SOCIETY
Volume: 15
Issue: 7
起始頁: 1426
結束頁: 1430
顯示於類別:期刊論文


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  1. 000267142400020.pdf