標題: Real-time molecular assessment on oxidative injury of single cells using Raman spectroscopy
作者: Chang, Wei-Tien
Lin, Hung-Lung
Chen, Hung-Che
Wu, Yao-Ming
Chen, Wen-Jone
Lee, Yuan-Teh
Liau, Ian
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
關鍵字: oxidative stress;reactive oxygen species;lipid peroxidation;laser tweezers;single cells
公開日期: 1-Sep-2009
摘要: Oxidative stress is encountered in many biological systems; the resultant oxidative injury plays a significant role in the pathogenesis of diverse diseases. Conventional measurements on oxidative injury are employed almost exclusively on a large population of cells either by counting the fraction of cell death or by observing the fluorometric change resulting from exogenous reagents, thereby lacking in molecular detail and temporal specificity. In this work we combine laser tweezers and Raman spectroscopy to observe the response of single cells to oxidative stress. By measuring the temporal changes of vibrational spectra of single optically trapped cells, we demonstrate a molecular-level assessment of cellular oxidative injury in real time, both qualitatively and quantitatively, without the introduction of exogenous reagents. The main experimental findings are supported by the observation of Raman spectra of intermediates and downstream products. The abrogation of the above changes by ascorbic acid further illustrates the therapeutic effect of antioxidants against cellular oxidative injury. This approach is extensible to studies exploring the biochemical transformation of single cells or intracellular organelles in response to various chemical or physical stimuli. With the aid of 'molecular fingerprints', single-cell Raman spectroscopy exhibits a great potential for accessing the chemical aspects of cellular bioactivity, yielding insight into pathophysiological processes and assisting the development of novel therapeutic interventions against diseases. Copyright (C) 2009 John Wiley & Sons, Ltd.
URI: http://dx.doi.org/10.1002/jrs.2261
http://hdl.handle.net/11536/6721
ISSN: 0377-0486
DOI: 10.1002/jrs.2261
期刊: JOURNAL OF RAMAN SPECTROSCOPY
Volume: 40
Issue: 9
起始頁: 1194
結束頁: 1199
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