標題: A rapid and selective method for monitoring the growth of coliforms in milk using the combination of amperometric sensor and reducing of methylene blue
作者: Lee, Yuan-Geuy
Wu, Hung-Yi
Hsu, Chuan-Liang
Liang, Hong-Jen
Yuan, Chiun-Jye
Jang, Hung-Der
生物科技學系
Department of Biological Science and Technology
關鍵字: Amperometric sensor;Methylene blue;Coliforms;Detection time
公開日期: 7-九月-2009
摘要: A novel method for monitoring the growth of coliforms in milk was developed based on measuring the current change in an amperometric sensor. The sensor consists of a circuit with a homemade potentiostat and a pair of electrodes. The electrode was immersed in milk samples containing methylene blue with various concentrations of bacterial inoculums. The microbial metabolism led to the reduction of methylene blue resulting in a change of current. The time required to identify readily detectable change (detection time, DT) provided an approximate measurement of the amount of microorganisms in the initial inoculums. The sensor system used in this study has the selectivity towards coliform bacteria such as Escherichia coli and Enterobacter aerogenes. The calibration curve of DT against concentration of coliform showed a linear correlation coefficient (R(2) = 0.9192) over the range of 10(2)-10(8) CFU/mL. The sensor was able to detect the coliform bacteria at initial concentrations of 10(5) CFU/mL within 6 h, making it suitable for use in real-time monitoring of bacterial growth. This system has potential application in the detection of coliform concentration in milk at dairy farms when a proper selective media is designed. (C) 2009 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.snb.2009.06.028
http://hdl.handle.net/11536/6686
ISSN: 0925-4005
DOI: 10.1016/j.snb.2009.06.028
期刊: SENSORS AND ACTUATORS B-CHEMICAL
Volume: 141
Issue: 2
起始頁: 575
結束頁: 580
顯示於類別:期刊論文


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