標題: Direct Observation of Single Molecule Conformational Change of Tight-Turn Paperclip DNA Triplex in Solution
作者: Liu, Ching-Ping
Wey, Ming-Tsai
Chang, Chia-Ching
Kan, Lou-Sing
生物科技學系
Department of Biological Science and Technology
關鍵字: DNA triplex;Atomic force microscopy;Tight-turn paperclip DNA;Single molecule image
公開日期: 1-Oct-2009
摘要: DNA triplex modulates gene expression by forming stable conformation in physiological condition. However, it is not feasible to observe this unique molecular structure of large molecule with 54 oligodeoxynucleotides directly by conventional nuclear magnetic approach. In this study, we observed directly single molecular images of paperclip DNA triplexes formation in a buffer solution of pH 6.0 by atomic force microscopy (AFM). Meanwhile, a diffuse "tail" of unwound DNA was observed in pH 8.0 solution. This designable approach in visualizing the overall structures and shapes of oligo-DNAs at the single molecular level, by AFM, is applicable to other biopolymers as well.
URI: http://dx.doi.org/10.1007/s12010-008-8390-1
http://hdl.handle.net/11536/6621
ISSN: 0273-2289
DOI: 10.1007/s12010-008-8390-1
期刊: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume: 159
Issue: 1
起始頁: 261
結束頁: 269
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