標題: Restriction mapping of genes by capillary electrophoresis with laser-induced fluorescence detection
作者: Peck, K
Wung, SL
Chang, GS
Yen, JJY
Hsieh, YZ
應用化學系
Department of Applied Chemistry
公開日期: 1-四月-1997
摘要: Restriction mapping is one of the essential steps in gene analysis and molecular biology studies, Slab gel electrophoresis is the traditional way to separate DNA fragments for restriction mapping, However, slab gel electrophoresis does not provide sufficient resolution as required in many mapping applications, and the use of radioisotopes in traditional mapping methods creates health hazards, In the present study, capillary electrophoresis coupled with laser-induced fluorescence detection and a modified partial digestion mapping procedure was developed to map DNA fragments. By using capillary electrophoresis, a restriction map of a genomic lambda phage clone of human interleukin 5 receptor a chain (IL5R alpha) gene was constructed, The IL5R alpha gene was analyzed to have five XbaI enzyme cutting sites at locations 1370, 2290, 2950, 5430, and 9330, The system was further characterized by using pBluescript SK(+) phagemid DNA as a model, Using a sequence-derived map as a reference, the pBluescript SK(+) restriction map constructed by capillary electrophoresis had an accuracy greater than 90%.
URI: http://hdl.handle.net/11536/617
ISSN: 0003-2700
期刊: ANALYTICAL CHEMISTRY
Volume: 69
Issue: 7
起始頁: 1380
結束頁: 1384
顯示於類別:期刊論文


文件中的檔案:

  1. A1997WQ77700024.pdf