標題: Primary events in the photodissociation of oxyhemoglobin
作者: Yabushita, Atsushi
Kobayashi, Takayoshi
電子物理學系
Department of Electrophysics
關鍵字: Ultrafast spectroscopy;hemoglobin;photo-dissociation
公開日期: 2010
摘要: The growing number of data on heme proteins in the ultrafast time domain have revealed complicated photophysics of the heme that are sensitive both to the structure of the protein and to the ligand species. In this work, ultrafast time-resolved pump-probe in visible spectral range was performed using an ultrashort visible laser pulse. Broad spectral width of visible laser pulse enabled us to observe the pump-probe signal with a broadband range. A broadband multi-channel detector array coupled to a multi-channel lock-in amplifier was used to obtain the pump-probe signals at all of the probe frequencies simultaneously. In the simultaneous measurement at many probe wavelengths, we could obtain ultrafast spectral change after the photo-excitation of oxy-hemoglobin only with a short measurement time, avoiding laser damage on the sample. The time constant of the primary process was determined for the first time. Ultrafast spectral change observed in the early delay time region directly shows ultrafast photo-dissociation process of oxy-hemoglobin.
URI: http://hdl.handle.net/11536/6136
http://dx.doi.org/10.3233/SPE-2010-0447
ISSN: 0712-4813
DOI: 10.3233/SPE-2010-0447
期刊: SPECTROSCOPY-AN INTERNATIONAL JOURNAL
Volume: 24
Issue: 3-4
起始頁: 333
結束頁: 338
顯示於類別:期刊論文


文件中的檔案:

  1. 000281667400023.pdf