標題: Mammalian Ste20-like protein kinase 3 induces a caspase-independent apoptotic pathway
作者: Lin, Chia-Ying
Wu, Hung-Yi
Wang, Pei-Ling
Yuan, Chiun-Jye
生物科技學系
Department of Biological Science and Technology
關鍵字: Mst3;Apoptosis;AIF;Endonuclease G;Mitochondria;DNase
公開日期: 1-一月-2010
摘要: In this study, it was shown that the mammalian sterile 20-like serine/threonine protein kinase 3 (Mst3) plays an essential role in the staurosporine-induced apoptosis of HeLa cells. The staurosporine-induced apoptosis was reduced by around 65% by the selective knockdown of Mst3 in stable clones, HeLa(siMst3). Although caspases were shown to be involved in the Mst3-mediated apoptosis, only 15-20% of staurosporine-induced apoptosis was suppressed by the caspase inhibitor, z-DEVD-fmk. Accordingly, Mst3 was proposed to trigger a caspase-independent apoptotic pathway in response to staurosporine. Interestingly, staurosporine greatly induced the mitochondrial membrane potential transition in HeLa cells, but had no effect in Hela(siMst3). The role of Mst3 in controlling the mitochondrial integrity was therefore proposed, presumably through the regulation of Bax. Furthermore, it was shown that staurosporine promoted the nuclear translocation of apoptosis-inducing factor and endonuclease G in HeLa cells. The nuclease activity associated with endonuclease G was also enhanced in response to staurosporine. However, both staurosporine-induced nuclear translocation of apoptosis-inducing factor and endonuclease G and the nuclease activity associated with endonuclease G were markedly reduced in Hela(siMst3). These results suggest that Mst3 may respond to staurosporine to trigger the caspase-independent apoptotic pathway by regulating the nuclear translocation of apoptosis-inducing factor and endonuclease G, and the nuclease activity associated with endonuclease G. (C) 2009 Elsevier Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.biocel.2009.09.012
http://hdl.handle.net/11536/6088
ISSN: 1357-2725
DOI: 10.1016/j.biocel.2009.09.012
期刊: INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
Volume: 42
Issue: 1
起始頁: 98
結束頁: 105
顯示於類別:期刊論文


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  1. 000273912200014.pdf