標題: Measurement of Macrophage Adhesion at Various pH Values by Optical Tweezers with Backward-Scattered Detection
作者: Su, Yi-Jr
Hsu, Long
電子物理學系
Department of Electrophysics
公開日期: 2010
摘要: Optical tweezers have emerged as a powerful tool with broad applications in biology and physics. In force-measuring applications, the trapped bead position is usually accurately determined by forward-scattered detection. The current study discusses both backward-scattered detection and forward-scattered detection related to the linear detection range for a 3 mm bead and the distance between the two laser system focuses, confirming the optimum positions of the two focuses. The result indicates that the linear detection range of backward-scattered detection is longer than the forward-scattered one. Finally, this work investigates real-time adhesion force measurements between human macrophages and 3 mm trapped beads coated with lipopolysaccharides at various pH values by optical tweezers with backward-scattered detection. (C) 2010 The Japan Society of Applied Physics
URI: http://hdl.handle.net/11536/6049
http://dx.doi.org/10.1143/JJAP.49.077002
ISSN: 0021-4922
DOI: 10.1143/JJAP.49.077002
期刊: JAPANESE JOURNAL OF APPLIED PHYSICS
Volume: 49
Issue: 7
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