標題: Preferential promotion of apoptosis of monocytes by Lactobacillus casei rhamnosus soluble factors
作者: Chiu, Yi-Han
Hsieh, Yi-Jen
Liao, Kuang-Wen
Peng, Kou-Cheng
生物科技學系
Department of Biological Science and Technology
關鍵字: Lactobacillus casei rhamnosus;Apoptosis;Monocytes;IBD;Mitochondrial pathway;TGF-beta
公開日期: 1-二月-2010
摘要: Background & aims: inflammatory bowel disease (IBD) is characterized by dense infiltrates of and defective apoptosis by mucosal cell populations. Some probiotics inhibit monocytes' expansion, although mechanisms remain unknown. Supernatants of Lactobacillus strains were investigated for inducing apoptosis of monocytes. Methods: Secreted factors produced by Latobacillus strains were tested on human lymphocytes, monocytes and a human monocytic leukemia-cell line (THP-1). Cell death mechanisms were investigated by a variety of methods. Lipopolysaccharide (LPS)-induced proinflammatory cytokines (IL-1 beta, IL-6, IL-8, TNF-alpha) and anti-inflammatory TGF-beta 1 were determined. Results: Soluble factor(s) from Lactobacillus casei rhamnosus strain supernatants (LcrS) effectively induced apoptosis of immune cells. These were mainly soluble proteins (MW 5-30 kDa; LcrS(5-30)). For immune cells, but not human colonic epithelial carcinoma cells (HT-29), pretreatment with LcrS(5-30) significantly promoted apoptosis via a mitochondrial pathway. LcrS(5-30) suppressed pro-inflammatory cytokines and induced anti-inflammatory TGF-beta 1. Conclusions: Probiotic Lcr produced heat-stable molecules (MW range 5-30 kDa) that promoted immune cell apoptosis without affecting intestinal epithelial cells. LcrS(5-30) triggered apoptosis by a mitochondrial pathway, but not via TGF-beta signaling pathway. LcrS(5-30) also inhibited LPS-induced inflammatory cytokines by immune cells. Thus, LcrS(5-30) promotes apoptosis of immune cells, and suggests probiotics-based regimens for prevention of IBD. (C) 2009 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
URI: http://dx.doi.org/10.1016/j.clnu.2009.07.004
http://hdl.handle.net/11536/5897
ISSN: 0261-5614
DOI: 10.1016/j.clnu.2009.07.004
期刊: CLINICAL NUTRITION
Volume: 29
Issue: 1
起始頁: 131
結束頁: 140
顯示於類別:期刊論文


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