標題: Alteration of the Substrate's Prefolded Conformation and Cyclization Stereochemistry of Oxidosqualene-Lanosterol Cyclase of Saccharomyces cerevisiae by Substitution at Phenylalanine 699
作者: Wu, Tung-Kung
Chang, Cheng-Hsiang
Wen, Hao-Yu
Liu, Yuan-Ting
Li, Wen-Hsuan
Wang, Tsai-Ting
Shie, Wen-Shiang
生物科技學系
Department of Biological Science and Technology
公開日期: 5-Feb-2010
摘要: The Saccharomyces cerevisiae ERG7(Phe699) mutants produced one chair-chair-chair (C-C-C) and two chair-boat-chair (C-B-C) truncated tricyclic compounds, one tetracyclic 17 alpha-exocyclic unrearranged intermediate, and two 17 beta-exocyclic truncated rearranged intermediates. These results provided direct evidence for the importance of the residue in affecting mechanistic transitions between C-B-C and C-C-C substrate conformation and between the 17 alpha- and 17 beta-exocyclic side chain stereochemistry as well as in stabilizing the 6 -6-5 tricyclic and the protosteryl C-17 cations.
URI: http://dx.doi.org/10.1021/ol902694y
http://hdl.handle.net/11536/5850
ISSN: 1523-7060
DOI: 10.1021/ol902694y
期刊: ORGANIC LETTERS
Volume: 12
Issue: 3
起始頁: 500
結束頁: 503
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