Title: Spatiotemporal Characterization of Phagocytic NADPH Oxidase and Oxidative Destruction of Intraphagosomal Organisms in Vivo Using Autofluorescence Imaging and Raman Microspectroscopy
Authors: Chang, Wei-Tien
Yang, Yi-Cyun
Lu, Hsueh-Han
Li, I-Lin
Liau, Ian
應用化學系
應用化學系分子科學碩博班
Department of Applied Chemistry
Institute of Molecular science
Issue Date: 17-Feb-2010
Abstract: The ability to generate antimicrobial reactive-oxygen species (ROS) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is critical for the host to defend against invading microbes. We have demonstrated the application of confocal autofluorescence microscopy and Raman microspectroscopy to characterize dynamically the phagocytic process of living macrophages in a label-free manner. In particular, we visualized the translocation of NADPH oxidases in livin macrophages that are undergoing phagocytosis of invading yeasts and monitored dynamically the change at the molecular level of single intraphagosomal yeasts caused by phagocytic ROS.
URI: http://dx.doi.org/10.1021/ja9086038
http://hdl.handle.net/11536/5830
ISSN: 0002-7863
DOI: 10.1021/ja9086038
Journal: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume: 132
Issue: 6
Begin Page: 1744
End Page: +
Appears in Collections:Articles


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