標題: Echinoid regulates Flamingo endocytosis to control ommatidial rotation in the Drosophila eye
作者: Ho, Yu-Huei
Lien, Mong-Ting
Lin, Chiao-Ming
Wei, Shu-Yi
Chang, Li-Hsun
Hsu, Jui-Chou
生物科技學系
Department of Biological Science and Technology
關鍵字: Echinoid;Flamingo (Starry night);DE-cadherin (Shotgun);Frizzled;Planar cell polarity;Endocytosis;Cell adhesion;Adherens junction;Drosophila
公開日期: 1-三月-2010
摘要: Planar cell polarity (PCP) refers to a second polarity axis orthogonal to the apicobasal axis in the plane of the epithelium. The molecular link between apicobasal polarity and PCP is largely unknown. During Drosophila eye development, differentiated photoreceptors form clusters that rotate independently of the surrounding interommatidial cells (ICs). Here, we demonstrate that both Echinoid (Ed), an adherens junction-associated cell adhesion molecule, and Flamingo (Fmi), a PCP determinant, are endocytosed via a clathrin-mediated pathway in ICs. Interestingly, we found that Ed binds the AP-2 adaptor and is required for the internalization of Fmi into ICs. Loss of ed led to increased amounts of Fmi on the cell membrane of non-rotating ICs and also to the misrotation of photoreceptor clusters. Importantly, overexpression of fmi in ICs alone was sufficient to cause misrotation of the adjacent photoreceptor clusters. Together, we propose that Ed, when internalized by AP-2, undergoes co-endocytosis with, and thereby decreases, Fmi levels on non-rotating ICs to permit correct rotation of ommatidial clusters. Thus, co-endocytosis of Ed and Fmi provides a link between apicobasal polarity and PCP.
URI: http://dx.doi.org/10.1242/dev.040238
http://hdl.handle.net/11536/5793
ISSN: 0950-1991
DOI: 10.1242/dev.040238
期刊: DEVELOPMENT
Volume: 137
Issue: 5
起始頁: 745
結束頁: 754
顯示於類別:期刊論文


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  1. 000274424600007.pdf