標題: Pulsed Electromagnetic Fields Accelerate Proliferation and Osteogenic Gene Expression in Human Bone Marrow Mesenchymal Stem Cells During Osteogenic Differentiation
作者: Sun, Li-Yi
Hsieh, Dean-Kuo
Lin, Po-Cheng
Chiu, Hsien-Tai
Chiou, Tzyy-Wen
生物科技學系
Department of Biological Science and Technology
關鍵字: pulsed electromagnetic fields;osteogenesis;mesenchymal stem cells
公開日期: 1-四月-2010
摘要: Osteogenesis is a complex series of events involving the differentiation of mesenchymal stem cells to generate new bone. In this study, we examined the effect of pulsed electromagnetic fields (PEMFs) on cell proliferation, alkaline phosphatase (ALP) activity, mineralization of the extracellular matrix, and gene expression in bone marrow mesenchymal stem cells (BMMSCs) during osteogenic differentiation. Exposure of BMMISCs to PEMFs increased cell proliferation by 29.6% compared to untreated cells at day 1 of differentiation. Semi-quantitative RT-PCR indicated that PEMFs significantly altered temporal expression of osteogenesis-related genes, including a 2.7-fold increase in expression of the key osteogenesis regulatory gene cbfa1, compared to untreated controls. In addition, exposure to PEMFs significantly increased ALP expression during the early stages of osteogenesis and substantially enhanced mineralization near the midpoint of osteogenesis. These results suggest that PEMFs enhance early cell proliferation in B M MSC-mediated osteogenesis, and accelerate the osteogenesis. Bioelectromatmetics 31:209-219, 2010. (C) 2009 Wiley-Liss, Inc.
URI: http://dx.doi.org/10.1002/bem.20550
http://hdl.handle.net/11536/5570
ISSN: 0197-8462
DOI: 10.1002/bem.20550
期刊: BIOELECTROMAGNETICS
Volume: 31
Issue: 3
起始頁: 209
結束頁: 219
顯示於類別:期刊論文


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