Separation of Benzodiazepines by Non-Aqueous Capillary Electrophoresis and Sweeping-Micellar Electrokinetic Chromatography
|關鍵字:||苯二氮平;非水相毛細管電泳法;掃掠式微胞電動層析法;毛細管電泳法;線上濃縮;疏水性藥物;benzodiazepines;non-aqueous capillary electrophoresis;sweeping in micellar electrokinetic chromatography;sweeping;capillary electrophoresis;on-line concentration|
使用非水相電泳可改善傳統水溶液電泳對於親油性藥物無法有效分離之問題，以非水相溶劑取代水進行電泳實驗可增進疏水性藥物之分離選擇性。夲研究中，我們成功的開發出在10分鐘內分析了八種苯二氮平類藥物分析物的非水相毛細管電泳法，其分離緩衝溶液組成為甲醇-乙腈(95:5,v/v)混合溶劑，其中包含450 mM 草酸和2.5 mM 三乙胺，分析物遷移時間的RSD皆小於1.25% 。
In this study, non-aqueous capillary electrophoresis (NACE) method and on-line concentration by sweeping in micellar electrokinetic chromatography (MEKC) method were employed to analyze benzodiazepines. The benzodiazepines are used clinically as sedatives to counteract anxiety. These compounds are frequently prescribed for pharmacotherapy of epilepsy, convulsion and many psychiatric disorders. They have been prescribed as muscle relaxants, tranquilizer and anticonvulsants. Benzodiazepines can lead to sudden death if misused, and have been the subject of abuse in suicide and criminal cases. The application of CE for the analysis of pharmaceutical drugs is limited because these drugs are hydrophobic and conventional aqueous CE methods do not generate useful separation. Using completely non-aqueous separation media instead of water as the CE buffer solvent allow improved selectivity. A successful separation of eight benzodiazepines drugs was completed within 10 min in a methanol-acetonitrile (95:5) buffer containing 450mM Oxalic acid and 2.5mM Triethylamine. The RSDs of migration times were less than 1.25%. However, the small sample volume and short optical length restricted the detection limits of CE. Thus, On-line concentration by sweeping in electrokinetic chromatography method was also used to improve the detection limits of benzodiazepines. The limits of detection were reduced to ppb level by the on-line concentration method. The highest response improvement was over 300-fold.
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