Title: Determination of cinnamaldehyde, cinnamic acid, paeoniflorin, glycyrrhizin and [6]-gingerol in the traditional Chinese medicinal preparation Kuei-chih-tang by cyclodextrin-modified micellar electrokinetic chromatography
Authors: Huang, HY
Kuo, KL
Hsieh, YZ
應用化學系
Department of Applied Chemistry
Keywords: Kuei-chih-tang;pharmaceutical analysis;cinnamaldehyde;cinnamic acid;paeoniflorin;glycyrrhizin;gingerol
Issue Date: 30-May-1997
Abstract: This study presents a cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) technique with a multi-wavelength detection method to determine specific components in Kuei-chih-tang. Kuei-chih-tang, a Chinese medicinal preparation, is composed of five crude herbs, i.e., Cinnamomi ramulus, Paeoniae radix, Glycyrrhizae radix, Zingiberis rhizoma and Zizyphi fructus. The effects of SDS and gamma-cyclodextrin on the analytes' migration behavior were examined. The selected components were successfully separated within 10 min using a pH 10.0 borax-NaOH buffer containing 20 mM SDS and 2 mM gamma-cyclodextrin. The correlation coefficients of the linear calibration graphs for the analytes exceeded 0.993. Moreover, the optimized CD-MEKC method was employed to analyze four different Kuei-chih-tang samples. (C) 1997 Elsevier Science B.V.
URI: http://dx.doi.org/10.1016/S0021-9673(97)00136-2
http://hdl.handle.net/11536/529
ISSN: 0021-9673
DOI: 10.1016/S0021-9673(97)00136-2
Journal: JOURNAL OF CHROMATOGRAPHY A
Volume: 771
Issue: 1-2
Begin Page: 267
End Page: 274
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