標題: Effects of static magnetic fields on the development and aging of Caenorhabditis elegans
作者: Hung, Yao-Ching
Lee, Jia-Huey
Chen, Huang-Meng
Huang, Guewha Steven
材料科學與工程學系奈米科技碩博班
Graduate Program of Nanotechnology , Department of Materials Science and Engineering
關鍵字: aging;C. elegans;static magnetic fields;life cycle
公開日期: 15-六月-2010
摘要: The current study investigated the possible effects of static magnetic fields (SMFs) on the developmental and aging processes of Caenorhabditis elegans. Nematodes were grown in the presence of SMFs of strengths varying from 0 to 200 mT. The rate of development and the lifespan were recorded. Treatment with a 200 mT SMF reduced the development time from the L2 to the L3 stage by 20%, from L3 to L4 by 23%, and from L4 to young adult by 31%. After SMF treatment, the average lifespan was reduced from 31 days to 24 days for wild-type nematodes. The up-regulation of clk-1, lim-7, daf-2, unc-3 and age-1 after SMF treatment was verified by quantitative real-time RT-PCR. Apparently, induction of gene expression is selective and dose dependent. The total developmental time was significantly reduced for the lin-4, lin-14, lin-41 and lim-7 mutants, but not for the let-7, clk-1, unc-3 and age-1 mutants. Lifespan analyses revealed that the let-7, unc-3 and age-1 mutants were not affected by SMF treatment. Here we show that SMFs accelerate nematode development and shorten nematode lifespan through pathways associated with let-7, clk-1, unc-3 and age-1.
URI: http://dx.doi.org/10.1242/jeb.039768
http://hdl.handle.net/11536/5262
ISSN: 0022-0949
DOI: 10.1242/jeb.039768
期刊: JOURNAL OF EXPERIMENTAL BIOLOGY
Volume: 213
Issue: 12
起始頁: 2079
結束頁: 2085
顯示於類別:期刊論文


文件中的檔案:

  1. 000278175700016.pdf