標題: Protein separation and enrichment by counter-current chromatography using reverse micelle solvent systems
作者: Shen, Ching-Wei
Yu, Tiing
應用化學系
Department of Applied Chemistry
關鍵字: counter-current chromatography;reverse micelle;protein separation;protein enrichment;pH gradient;ionic strength gradient
公開日期: 1-Jun-2007
摘要: A protein mixture consisting of myoglobin, cytochrome c, and lysozyme was separated by high-speed counter-current chromatography using a two-phase aqueous/reverse micelle-containing organic solvent system. About 50% stationary phase retention ratio was obtained in most chromatographic experiments. Separations were manipulated mainly by pH gradients that controlled the electrostatic interactions between the protein molecules and reverse micelles. Separations were further improved by incorporating an ionic strength gradient along with the pH gradient. Control of ionic strength in the aqueous solution helped fine-tune protein partitioning between the stationary and mobile phases. Although non-specific protein interactions affected baseline resolution, recovery of cytochrome c and lysozyme reached 90% and 82%. Furthermore, concentration or enrichment of these two proteins was achieved from a large-volume sample load. This technique can potentially be employed in the recovery and enrichment of proteins from large-volume aqueous solutions. (C) 2007 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.chroma.2007.01.079
http://hdl.handle.net/11536/4586
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2007.01.079
期刊: JOURNAL OF CHROMATOGRAPHY A
Volume: 1151
Issue: 1-2
起始頁: 164
結束頁: 168
Appears in Collections:Conferences Paper


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