標題: Monolithic integration of well-ordered nanoporous structures in the microfluidic channels for bioseparation
作者: Kuo, Chiung-Wen
Shiu, Jau-Ye
Wei, Kung Hwa
Chen, Peilin
材料科學與工程學系
Department of Materials Science and Engineering
關鍵字: colloidal crystal;DNA separation;microchip;SU-8;inverse opal;self-assembly
公開日期: 31-八月-2007
摘要: Gel electrophoresis and capillary gel electrophoresis are widely used for the separation of biomolecules. With increasing demand in the miniaturized devices such as lab-on-a-chip, it is necessary to integrate such a separation component into a chip format. Here, we describe a simple approach to fabricate robust three-dimensional periodic porous nanostructures inside the microchannels for the separation of DNA molecules. In our approach, the colloidal crystals were first grown inside the microchannel using evaporation assisted self-assembly process. Then the void spaces among the colloidal crystals were filled with epoxy-based negative tone photoresist (SU-8). UV radiation was used to cure the photoresist at the desired area inside the microchannel. After subsequent development and nanoparticle removal, the well-ordered nanoporous structures inside the microchannel were obtained. Our results indicated that it was possible to construct periodic porous nanostructures inside the microchannels with cavity size around 300 nm and interconnecting pores around 30 nm. The mobility of large DNA molecules with different sizes was measured as a function of the applied electric field in the nanoporous materials. It was also demonstrated that I kilo-base pair (kbp) DNA ladders could be separated in such an integrated system within 10 min under moderate electric field. (c) 2007 Published by Elsevier B.V.
URI: http://dx.doi.org/10.1016/j.chroma.2007.06.037
http://hdl.handle.net/11536/4263
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2007.06.037
期刊: JOURNAL OF CHROMATOGRAPHY A
Volume: 1162
Issue: 2
起始頁: 175
結束頁: 179
顯示於類別:會議論文


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