標題: An optical biosensing platform for proteinase activity using gold nanoparticles
作者: Chuang, Yao-Chen
Li, Jung-Chun
Chen, Sz-Hau
Liu, Ting-Yu
Kuo, Ching-Han
Huang, Wei-Ting
Lin, Chih-Sheng
生物科技學系
Department of Biological Science and Technology
關鍵字: 6-Mercaptohexan-1-ol;Gold nanoparticles;Matrix metalloproteinase;Optical biosensor;Proteinase
公開日期: 1-Aug-2010
摘要: The surface plasmon resonance (SPR) wavelength of colloidal gold nanoparticles (AuNPs) can vary when the AuNPs aggregate, have different sizes or shapes, or are modified with chemical molecules. In this study, an optical biosensing platform for a proteinase activity assay was established based on the SPR property of AuNPs. The 13-nm AuNPs were modified with gelatin (AuNPs-gelatin) as a proteinase substrate and subsequently modified with 6-mercaptohexan-1-ol (MCH) (AuNPs/MCH-gelatin). After proteinase (trypsin or gelatinase) digestion, the AuNPs lose shelter, and MCH increases the attractive force between the modified AuNPs. Therefore, the AuNPs gradually move closer to each other, resulting in AuNPs aggregation. The AuNPs aggregation can be monitored by the red shift of surface plasmon absorption and a visible color change of the AuNPs is from red to blue. Such a color change can be observed with the naked eye. For detection, the absorption ratio, A(625)/A(525), of the reacted AuNPs solution can be used to estimate quantitatively the proteinase activity. A linear correlation has been established with trypsin activity at concentrations from 1.25 x 10(-1) to 1.25 x 10(2) U and matrix metalloproteinase-2 activity at concentrations from 50 ng/mL to 600 ng/mL. (C) 2010 Elsevier Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.biomaterials.2010.04.026
http://hdl.handle.net/11536/32368
ISSN: 0142-9612
DOI: 10.1016/j.biomaterials.2010.04.026
期刊: BIOMATERIALS
Volume: 31
Issue: 23
起始頁: 6087
結束頁: 6095
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